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DOI :
10.3791/61027-v
•
9:15 min
May 9th, 2020
Chapters
0:04
Introduction
0:58
UV Crosslinking and Lysate Production
3:18
Elution and SDS-PAGE
5:24
PCR and Gel Extraction
7:06
Results: Temporal Binding of Nrd1 to its Targets
8:47
Conclusion
cDNA的动力学交联和分析是一种允许以高时间分辨率研究活细胞中蛋白质-RNA相互作用动力学的方法。这里详细描述了该方案,包括酵母细胞的生长、UV交联、收获、蛋白质纯化和下一代测序文库的制备步骤。
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