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5.6K Views
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04:36 min
June 12th, 2020
DOI :
10.3791/61074-v
Chapters
0:04
Introduction
0:40
Device and Animal Preparation
1:13
Mouse and Electrode Platform Setup
2:38
Results: Representative Electrocardiogram (ECG) Reads and Analyses
3:51
Conclusion
Transcript
这种独特的协议允许使用心电图技术评估新生儿小鼠心血管功能立即以非侵入性的方式,而无需使用麻醉剂。评估产后早期心血管功能将扩大我们对新生儿心脏的理解,其方式可与人类临床数据直接相关。要准备用于分析的测量设备,请将设备插入计算机的 USB 端口。
测量设备将自动开始加热至 37 摄氏度。大约15分钟后,将一只老鼠幼崽从家庭笼子里取出,然后用70%乙醇擦拭胸部。将幼崽放在塑料的加热表面上,让动物在黑暗中适应表面约两到五分钟。
当鼠标正在适应时,收集一小滴胶粘剂导电凝胶,然
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Summary
在这里,我们提出了一个非侵入性心电图(ECG)协议,针对早期产后小鼠进行了优化,不需要使用麻醉剂。
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