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A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

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07:51 min

June 3rd, 2020

June 3rd, 2020

6,958 Views

0:04

Introduction

0:35

Growing Germ-free Cabbages

3:03

Inoculating the Phyllosphere with Microbial Solutions

4:56

Preparing Sterile Vegetable Extract

5:59

Results: Growth of Microbial Inoculates in the Napa Cabbage Phyllosphere and Sterile Vegetable Extract

7:20

Conclusion

Transcript

Using this protocol, we can address key questions regarding how microbes assemble and interact in the phyllosphere. It has the added benefit that by using the sterile vegetable extract, we can link the structure of phyllo sphere communities to the

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A method of growing germ-free Napa cabbages has been developed which enables researchers to evaluate how single microbial species or multispecies microbial communities interact on cabbage leaf surfaces. A sterile vegetable extract is also presented which can be used to measure shifts in community composition during vegetable fermentation.

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