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DOI :
10.3791/61165-v
•
9:42 min
May 16th, 2020
Chapters
0:04
Introduction
0:57
Zebrafish Larva Hindbrain Ventricle Microinjection
4:21
Establishment of Injected and Viable Spore Numbers
5:22
Drug Treatment of Larvae and Survival Analysis
5:53
Zebrafish Wounding and Entrapment Device for Growth and Imaging
7:23
Results: Infection Outcome of Aspergillus Spores Microinjected into Zebrafish Larvae
9:07
Conclusion
该协议描述了斑马鱼幼虫的 阿斯珀吉勒斯 感染模型。 孢 子被微注入幼虫的后脑,化学处理用于诱导免疫抑制。感染进展通过每日成像设置进行监测,以监测真菌生长和免疫反应,以及通过菌落形成单位电镀来列举活孢子。
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