Preparation of the Meninges and CNS Tissues for Flow Cytometry Staining
4:33
Preparation of Single‐Cell Suspensions of Brain and Spinal Cord Tissue
6:57
Results: Immune Cells in CNS Tissues of TMEV‐IDD Mice
8:50
Conclusion
Transcript
Rapid isolation of CNS tissues including the meninges allows for a comprehensive analysis of immune cell phenotype, function, and localization in homeostatic and pathogenic conditions. The two demonstrated methods allow for the quick extraction of
Sign in or start your free trial to access this content
Dette papir præsenterer to optimerede protokoller til undersøgelse af residente og perifert afledte immunceller i centralnervesystemet, herunder hjernen, rygmarven og meninges. Hver af disse protokoller hjælper med at fastslå funktionen og sammensætningen af cellerne, der optager disse rum under steady state og inflammatoriske tilstande.