JoVE Logo
Faculty Resource Center

Sign In

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

DOI :

10.3791/61196-v

June 13th, 2020

June 13th, 2020

5,888 Views

1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Department of Biology and Chemistry, University of Bremen

Presented here is a protocol to monitor degradation of the protein huntingtin fused to the photoconvertible fluorophore Dendra2.

Tags

In Vivo

-- Views

Related Videos

article

Generation of Stable Transgenic C. elegans Using Microinjection

article

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

article

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

article

Antibody Staining in C. Elegans Using "Freeze-Cracking"

article

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

article

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

article

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

article

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

article

Measurements of Physiological Stress Responses in C. Elegans

article

Measuring RAN Peptide Toxicity in C. elegans

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved