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JoVE Journal

Biochemistry

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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

7.1K Views

06:10 min

June 18th, 2020

June 18th, 2020

7,097 Views

0:04

Introduction

0:46

Cell Preparation

1:33

Adenoviral Redox Sensitive Green Fluorescent Protein (roGFP) Transduction

2:46

Cysteine/Cystine (CyS/CySS) Balance Acquisition

4:01

Results: Representative Cellular Oxidation Evaluation

5:29

Conclusion

Transcript

Current protocol assessors in textile, redox state changes in an intracellular compartment-specific manner. Our protocol enables a better understanding and monitoring all the physiological or pathophysiological mechanisms of oxidative stress. This

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This protocol describes the assessment of subcellular compartment-specific redox status within the cell. A redox-sensitive fluorescent probe allows convenient ratiometric analysis in intact cells.

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