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Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications Video (Video) | JoVE

DOI :

10.3791/61230-v

June 12th, 2020

June 12th, 2020

9,423 Views

1Departments of Medicine, Albert Einstein College of Medicine, 2Departments of Molecular Pharmacology, Albert Einstein College of Medicine, 3Fleischer Institute of Diabetes and Metabolism

This publication describes a protocol for the isolation of nuclei from mature adipocytes, purification by fluorescence-activated sorting, and single-cell level transcriptomics.

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