Representative Images of Larval and Adult Optic Lobes
10:47
Conclusion
Transcript
Describimos un protocolo para diseccionar, inmunoteñir y montar cerebros de larvas y adultos de Drosophila con especial énfasis en los lóbulos ópticos. Dada la compleja organización tridimensional del lóbulo óptico, se anticipa que el protocolo de
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This protocol describes three steps to prepare larval and adult Drosophila optic lobes for imaging: 1) brain dissections, 2) immunohistochemistry and 3) mounting. Emphasis is placed on step 3, as distinct mounting orientations are required to visualize specific optic lobe structures.