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4.1K Views
•
10:32 min
June 10th, 2020
DOI :
10.3791/61365-v
Chapters
0:04
Introduction
0:30
Chlamydia trachomatis (Ctr) Reporter Mutagenesis
1:34
Mutant Ctr Imaging
3:09
Microscope Setup and Imaging
4:25
Altered Developmental Mutagenized Chlamydia Phenotype Identification and Isolation
7:07
Mutant Isolate Phenotype Verification
8:06
Results: Representative Ctr Developmental Mutant Identification
9:52
Conclusion
Transcript
该协议为具有改变发育特征的衣原体的生成和分离提供了一个独特的工具,最终允许识别调节发育周期的基因。这种方法的主要优点是能够实时跟踪衣原体细胞类型的发展,以及通过改变发育方案来分离衣原体的能力。为了使沙眼衣原体变化,记者看到一种衣原体,里面含有大约三倍于第十至第七的小学体。
与记者的血浆的兴趣和颗粒转换, 思想是库存的中分。使用10%功率的声波10秒,在100微升的意外代谢缓冲液中重新悬浮基本体,并在两个1.5毫升的微型离心管之间均等地分割由此产生的基本体悬浮液。将50微升新鲜准
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Summary
该协议利用荧光启动子-记者,活细胞显微镜,和个人包含提取在定向前的基因方法,以识别和分离 衣原体沙眼的发育突变体。
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