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DOI :
10.3791/61368-v
•
9:06 min
September 18th, 2020
Chapters
0:04
Introduction
0:47
Exopher Detection
2:11
Touch Neuron Identification
4:26
Exopher Identification and Scoring
6:43
Non-Exopher Identification
7:13
Results: Representative Exopher Production
8:26
Conclusion
该协议描述了检测和定量由 C.elegans细胞以膜结合 外外层形式产生的大型聚集和/或细胞器挤出(±4 μm)的方法。我们描述了菌株、生长条件、评分标准、时间和显微镜注意事项,以便于解剖这种碎片驱逐机制。
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