Results: E14 Differentiation into EBs, NPCs, and Neurons
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Conclusion
Transcript
In addition to the protocol for mouse embryonic stem cell differentiation into neuron cells, we also show a set of experiments that can comprehensively analyze the differentiation process. These techniques are pertinent to early ectodermal embryon
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Descriviamo la procedura per la differenziazione in vitro di cellule staminali embrionali di topo in cellule neuronali utilizzando il metodo della goccia sospesa. Inoltre, eseguiamo un'analisi fenotipica completa attraverso RT-qPCR, immunofluorescenza, RNA-seq e citometria a flusso.