A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

1:24

Identify Potential Genetic Interactions for Screening

2:35

Design the Cloning Strategy to Clone SIR2 into the pRS315 Vector

5:46

Transform the Vector into atg1△ and Wild Type Yeast Strains

7:32

Determine the Chronological Life Span to Test for Shortened CLS Phenotype Suppression

8:13

Results: Chronological Lifespan of atg1△+pRS315-SIR2

10:07

Conclusion