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2.7K Views
•
09:46 min
July 8th, 2021
DOI :
10.3791/61512-v
Chapters
0:05
Introduction
1:12
Pellet Preparation and Soluble Biomaterial Extracts Acquisition
2:11
Morphology Evaluation
3:28
Cell Function Assessment
5:54
Mineralized Formation Assessment
6:50
Results: Cell Viability, Morphology, and Protein Expression in Response to Biomaterial Exposure
8:30
Conclusion
Transcript
随着用于医疗应用的新材料的爆炸式增长,应系统地进行初步筛选,因此该协议可以成为旨在开发和表征新型生物材料的研究人员的基本工具。该方法允许定量和定性评估生物材料和药物制剂(固体和液体)的细胞毒性和生物活性。细胞暴露后,可以进行几种互补测定。
生物材料细胞毒性的建立可能对临床实践产生相关意义。例如,这些知识可以使临床医生能够为特定患者或病症选择最合适的生物材料。该协议允许在不同领域表征新型生物材料,例如牙科,骨科,外科,眼科,心脏病学等。
评估性能应允许得出除细胞毒性外
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Summary
该方法旨在通过使用活力测定和表型分析(包括流式细胞术、RT-PCR、免疫细胞化学以及其他细胞和分子生物学技术)制备可溶性提取物来评估生物材料的细胞毒性。
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