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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

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Young inflorescences as explant for callus production are important for establishing a rapid and efficient gene transformation and a regeneration method for revealing gene function as well as for crop improvement. Young inflorescences as explant f

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This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in rice.

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