Results: Representative Spermatogenic Cell Imaging, Yield, and Viability
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Conclusion
Transcript
This new efficient method for isolating subpopulations of spermatocytes and spermatids from adult mice can be used to investigate the molecular mechanisms underlying meiosis and spermatogenesis. This method uses a low cytotoxicity DNA binding dye
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Here we present a simple and efficient method to isolate live meiotic and post-meiotic germ cells from adult mouse testes. Using a low-cytotoxicity, violet-excited DNA binding dye and fluorescence-activated cell sorting, one can isolate highly enriched spermatogenic cell populations for many downstream applications.