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DOI :
10.3791/61704-v
August 28th, 2020
Chapters
0:04
Introduction
0:41
Transcardial Perfusion
1:59
Brain Extraction
4:44
Local Field Potential (LFP) Spontaneous Activity Recording
6:04
Results: Representative Spontaneous Sharp-Wave Ripple (SWR) Recordings
7:17
Conclusion
此协议描述了从表现出自发锐波波纹活动的小鼠身上制作水平海马-内皮层 (HEC) 切片的准备工作。切片在简化的接口夹腔中孵育,并在水下条件下用快速流动的人工脑脊液进行录音,以促进组织氧合和网络水平活动的自发出现。
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