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10.1K Views
•
07:24 min
February 6th, 2021
DOI :
10.3791/61749-v
Chapters
0:04
Introduction
0:54
Bacillus cereus Culture and Dilution
1:48
Mouse Intravitreal Injection
4:02
Eye Harvest
4:43
Intraocular Bacterial Count
6:06
Results: Representative Bacterial Endophthalmitis Mouse Model Validation
6:47
Conclusion
Transcript
我们的协议允许将微生物和治疗剂可重复引入小鼠眼部,以研究眼内感染的发病机制和新颖的治疗效果。我们的技术允许在小鼠模型中建立眼内感染,并有助于微生物的定量、宿主免疫反应、感染相关的宿主和病原体基因表达以及新颖的治疗效果。注射部位、针角和分娩的可视化对于成功执行此技术至关重要,适当的眼部收获对于感染参数的量化至关重要。
在生物安全二级设施中,将五毫升 BHI 肉汤添加到 10 毫升的扣盖管中,并使用无菌回路将单个 B.cereus 菌落从螺旋板培养物转移到 5 毫升肉汤中。短暂涡旋
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Summary
在这里,我们描述了一种细菌性心内膜炎小鼠模型中的内注射和随后的细菌定量方法。该协议可以扩展用于测量宿主免疫反应和细菌和宿主基因表达。
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