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2.8K Views
•
06:31 min
February 5th, 2021
DOI :
10.3791/61772-v
Chapters
0:04
Introduction
0:45
Preparation
1:37
Laparotomy
2:12
Tracheal Occlusion
3:47
Harvest
4:13
Histology and Tissue Processing for Protein and DNA Analyses
5:06
Results: Histological Analyses of the Fetal Lung Tissue Dissected Post TO
5:54
Conclusion
Transcript
这项技术最重要的优点是可逆性、降低动物成本和维护费用以及缩短怀孕期。此外,动物死亡率降低,因为不需要子宫切除术。基因研究的机会也更大。
该技术可用于研究先天性隔膜气喘的病理生理学。它也可以应用于了解肺部的其他疾病,如奶牛和肺发育不良。首先允许老鼠在下午6点到次日上午9点在同一个笼子里交配。
要确定E0,观察阴道塞,它有一个同质的,外带连接到阴道壁和内区是纤维,包括一些精子,形成纠缠质量与插头材料的纤维混合。记录小鼠在交配时和E10上的重量,以确保持续怀孕,并在E16
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Summary
各种先天性隔膜气喘和胎儿气管闭塞的动物模型在伦理问题、成本、手术难度、大小、存活率和遗传工具的可用性方面都存在利弊。该模型为研究气管闭塞和增加的发光压力对肺发育的影响提供了新的工具。
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