Breast Cancer Cell Culture Single Cell Suspension Generation
1:22
Tissue Sample Single Cell Suspension Generation
2:45
Colony Forming Assay
3:54
Mammosphere Assay and 3D Culture Model
5:09
Results: Representative BCSC Identification and Functional Evaluation
6:28
Conclusion
Transcript
This technique will allow researchers to isolate breast cancer stem cells at maximum purity and viability for the performance of both in vitro and in vivo functional characterization assays. Isolated breast cancer stem cells can also be used to he
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Este protocolo experimental describe el aislamiento de BCSC a partir de muestras de células y tejidos de cáncer de mama, así como los ensayos in vitro e in vivo que se pueden utilizar para evaluar el fenotipo y la función de BCSC.