Results: TCRβ and IGH Immune Repertoire Data Analysis
8:27
Conclusion
Transcript
This method enables exploration of specific T and B cells at the nucleotide or amino acid level and thus can identify dynamic changes in lymphocyte populations and diversity parameters in different disorders. The advantage of next-generation seque
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The current protocol describes a method for DNA isolation from blood samples and intestinal biopsies, generation of TCRβ and IGH PCR libraries for next-generation sequencing, performance of a NGS run and basic data analysis.