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DOI :
10.3791/61821-v
December 23rd, 2020
Chapters
0:05
Introduction
3:04
CTB-Cholera Toxin Subunit B Labeling and Antibody Application
5:02
Mounting the Sample for Imaging
7:39
Conclusion
0:46
Sample Preparation
1:36
Dehydration and Depigmentation
4:17
Tissue Clearing
5:59
Results: Confocal Images of the Hydrophobically Cleared Tissue Samples with Fluorescent Labelled TH Neurons and CTB Markers
ここでは、インタクトな脳構造の一部として標的分子を観察することを可能にする疎水性組織クリアリング法を提示する。この技術は現在、両方の男女のF344/ NコントロールおよびHIV-1トランスジェニックラットについて検証されている。
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