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DOI :
10.3791/61884-v
October 27th, 2020
Chapters
0:00
Introduction
0:46
Visceral Adipose Tissue Collection and Enzymatic Digestion
2:10
RNA Extraction from Mature Adipocytes and microRNA Quantification Assay
3:16
Characterization of Macrophage Subsets from Stromal Vascular Fraction
4:42
Results: Identification of Macrophages from Visceral Adipose Tissue
6:02
Conclusion
该方案提供了一种高效的胶原酶消化方法,用于在单个过程中从人内脏脂肪中分离活脂肪细胞和基质血管部分-SVF细胞,包括从脂肪细胞中获得高质量RNA的方法,以及通过对多种膜结合标记物进行染色以通过流式细胞术分析的SVF-巨噬细胞表型。
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