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DOI :
10.3791/61892-v
December 4th, 2020
Chapters
0:04
Introduction
1:04
Nasonia Rearing
2:19
Alignment of White and Black Pupae
3:21
Needle Preparation
3:53
Adult Injection with Aspirator Tube
5:04
6:32
Post-Injection Care and Mutant Screening
7:27
Results: Survival and Efficiency Rate of RNAi and CRISPR/Cas9 via Pupal and Adult Injections
7:52
Conclusion
在这里,我们将 纳索尼亚维特里彭尼斯 高效注射 pupal 和成人注射的方法描述为胚胎微注射的可访问替代品,通过 RNA 干扰 (RNAi) 或通过 CRISPR/Cas9 基因组编辑进行基因敲除,实现感兴趣的基因的功能分析。
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