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2.3K Views
•
08:30 min
February 9th, 2021
DOI :
10.3791/61930-v
Chapters
0:05
Introduction
0:40
Regular Maintenance of Harpegnathos saltator Colonies
2:05
Preparation of Quartz Glass Microinjection Needles and Microinjector
4:10
Injection of Embryos
6:32
Results: Mutagenesis Induction in H. saltator Ants
7:37
Conclusion
Transcript
该协议使我们能够生成转基因蚂蚁,以了解基因在社会环境中的交流和行为中的功能。操纵真社会昆虫的遗传系统具有挑战性,因为许多物种不会在实验室环境中交配和繁殖。蚂蚁高鼻细胞底中引人注目的表型可塑性使我们能够建立CRISPR介导的突变系。
演示该程序的将是来自实验室的研究生Kayli Sieber。在22至25摄氏度的蚂蚁饲养室的透明塑料盒中保持野生型H.saltator菌落,并有12小时的光照,12小时的黑暗照明时间表。使用小箱子饲养单个工蜂或小菌落,使用中型或大型箱子饲养较大的菌落
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Summary
昆虫真社会性的许多特征依赖于殖民地内的交流和分工。通过显微注射和CRISPR介导的诱变对蚂蚁胚胎中关键调控基因的遗传操作为真社会昆虫利他行为的本质提供了见解。
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