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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

DOI :

10.3791/62001-v

7:46 min

December 11th, 2020

December 11th, 2020

4,869 Views

1Department of Chemistry and Biochemistry, The University of Tulsa

The goal of this protocol is to enable seamless CRISPR/Cas9 editing of the C. elegans genome using assembled ribonucleoprotein complexes and the dpy-10 co-CRISPR marker for screening. This protocol can be used to make a variety of genetic modifications in C. elegans including insertions, deletions, gene replacements and codon substitutions.

Tags

CRISPR Cas9 Editing

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