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JoVE Journal

Biochemistry

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Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

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08:33 min

April 11th, 2021

April 11th, 2021

4,244 Views

0:00

Introduction

0:39

Preparation of Yeast Culture

2:10

Radioactive Isotope Incorporation

3:34

Yeast Cell Lysis and Extraction of Proteins

4:20

Precipitation of Proteins

5:15

Electrophoretic Separation

5:43

Autoradiography

5:59

Representative Results

7:56

Conclusion

Transcript

Mitochondria are essential organelles of eukaryotic cells capable for aerobic respiration. Their dysfunction is a well known source of human disease. Baker's yeast mitochondria contains circular genome encoding eight proteins.

In this video,

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Baker’s yeast mitochondrial genome encodes eight polypeptides. The goal of the current protocol is to label all of them and subsequently visualize them as separate bands.

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