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07:03 min
February 3rd, 2021
DOI :
10.3791/62077-v
Chapters
0:05
Introduction
0:41
Intravenous Injection of Cancer Cells for Metastatic Colony Formation
3:06
Embryo Maintenance During the Metastatic Colony Growth and their Isolation
5:26
Results: Metastatic Colony Isolation for Different Steps of the Screening Protocol
6:34
Conclusion
Transcript
我们的协议允许有效地识别基因或基因组合,阻止癌症入侵的观众。过度养殖不需要先进的动物设施进行维护。整体而言,作为一般或一般的特殊图书馆,可以在几周内在观众中放映。
这种过度筛选系统将帮助研究人员发现新的治疗基因靶点或基因靶点组合,可用于阻止癌症转移。对于 IV 注射,将细胞浓缩到每毫升 0.5 到 100 万细胞,使用冰冷 1X PBS 稀释或重新悬浮细胞。预计每10个胚胎需要大约1毫升的细胞悬浮。
要组装注射装置,将针头安装到注射器上,然后用三到五厘米长的管子将注
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Summary
这是一种有效的方法来筛选抑制剂或癌症转移的驱动因素。细胞,用表达库转导,被注射到鸡胆碱膜血管,形成转移菌落。已降低或增加侵入性的菌落被切除、扩大、重新注入以确认其表型,最后使用高吞吐量测序进行分析。
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