Name: rapid model to evaluate the anti-obesity potential of a combination of syzygium aromaticum (clove) and cuminun cyminum (cumin) on c57bl6/j mice fed high-fat diet
Uploaded: 2021-07-31T13:00:00
Description: Watch this Scientific Journal Video about Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum Clove and Cuminun cyminum Cumin on C57BL6/j Mice Fed High-Fat Diet

This research was supported by Instituto Polit&#233;cnico Nacional M&#233;xico.

All animal experiments were approved by the Animal Experiment Committee of Escuela Nacional de Ciencias Biologicas (IPN; Protocol No. 2732).
1. Preparation of the extract of the combination of cloves and cumin
NOTE: The edible plants, Syzygium aromaticum (clove) and Cuminum cyminum (cumin) were purchased from Central de Abastos CDMX, Mexico.
<ol>
	<li>Grind 500 g of seeds from the plants and perform ultrasonic-assisted extraction. Sonicate them (...

<ol>
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Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Syzygium+aromaticum+ethanol+extract+reduces+high-fat+diet-induced+obesity+in+mice+through+downregulation+of+adipogenic+and+lipogenic+gene+expression.">Syzygium aromaticum ethanol extract reduces high-fat diet-induced obesity in mice through downregulation of adipogenic and lipogenic gene expression.</a> Experimental and Therapeutic Medicine. 4 (3), 409-414 (2012).</li><li>Said, O., Saad, B., Fulder, S., Khalil, K., Kassis, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Weight+Loss+in+Animals+and+Humans+Treated+with+"Weigh+level",+a+Combination+of+Four+Medicinal+Plants+Used+in+Traditional+Arabic+and+Islamic+Medicine.">Weight Loss in Animals and Humans Treated with "Weigh level", a Combination of Four Medicinal Plants Used in Traditional Arabic and Islamic Medicine.</a> Evidence-Based Complementary and Alternative Medicine. , 874538 (2011).</li><li>Anaya-Esparza, L. M., Ramos-Aguirre, D., Zamora-Gasga, V. M., Yahia, E., Montalvo-Gonzalez, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+ultrasonic-assisted+extraction+of+phenolic+compounds+from+Justicia+spicigera+leaves.">Optimization of ultrasonic-assisted extraction of phenolic compounds from Justicia spicigera leaves.</a> Food Science and Biotechnology. 27 (4), 1093-1102 (2018).</li><li>Ayoub, R. 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In this study we evaluated, for the first time, the effect of oral administration of a combination of cloves and cumin (CC) extract on lipid profiles and obesity in mice fed with a high-fat diet for 5 weeks. Findings indicated that the HFD groups showed a significantly higher body weight gain compared to the ND group, which showed that the induction of obesity in the obese model was successful. The administered dose of CC (100, 200 and 450 mg/kg/day) produced a reduction in food intake and body weight. Consequently, the ...

Excess body fat accumulation is a characteristic of obesity. The disequilibrium between energy intake and consumption leads to the storage of excess energy in adipocytes, which is related to metabolic risk factors for hyperglycemia and insulin resistance in type 2 diabetes, hypertension, hypercholesterolemia, and cardiovascular disease1,2.
Natural products with minimum side effects and low cost have received increased attention since previous studies have reported bioactive phytochemicals and potential anti-obesity agents with mechanisms that reverse or delay metabolic syndrome and associated pathologies8.
Several medicinal plants have been studied to prevent obesity and related diseases. Among them, Syzygium aromaticum has been investigated for its anti-obesity potential in in vitro treatment on 3T3-L1 cells and in vivo treatment on mice fed with a high-fat diet11.&#160;In addition, significant anti-overweight effects were observed in a multi-center open trial of Cuminum cyminum on extremely obese subjects12. In this study, C57BL6/J mice was used to investigate the experimental fast model to evaluate a potential anti-obesity agent using a combination of both edible plants.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Distilled Water</td>
			<td>Any vendor</td>
			<td>n/a</td>
			<td>Available for other vendors as well</td>
		</tr>
		<tr>
			<td>Ethanol</td>
			<td>Fermont</td>
			<td>6063</td>
			<td>99.8% purity</td>
		</tr>
		<tr>
			<td colspan="4">&#160;Diet Ingredients</td>
		</tr>
		<tr>
			<td>Casein</td>
			<td>Any vendor</td>
			<td>n/a</td>
			<td></td>
		</tr>
		<tr>
			<td>Cellulose</td>
			<td>Any vendor</td>
			<td>n/a</td>
			<td></td>
		</tr>
		<tr>
			<td>Centrum balance multivitamin</td>
			<td>Pfizer</td>
			<td>n/a</td>
			<td></td>
		</tr>
		<tr>
			<td>Choline Bitartrate</td>
			<td>Any vendor</td>
			<td>n/a</td>
			<td></td>
		</tr>
		<tr>
			<td>L- cystein</td>
			<td>Sigma Aldrich</td>
			<td>168149</td>
			<td>Available for other vendors as well</td>
		</tr>
		<tr>
			<td>Lard</td>
			<td>Any vendor</td>
			<td>n/a</td>
			<td></td>
		</tr>
		<tr>
			<td>Maltodextrin 10</td>
			<td>Any vendor</td>
			<td>n/a</td>
			<td></td>
		</tr>
		<tr>
			<td>Pellets Nutricubos</td>
			<td>Purina</td>
			<td>n/a</td>
			<td>Available for other vendors as well</td>
		</tr>
		<tr>
			<td>Soybean Oil</td>
			<td>Any vendor</td>
			<td>n/a</td>
			<td></td>
		</tr>
		<tr>
			<td>Sucrose</td>
			<td>Any vendor</td>
			<td>n/a</td>
			<td></td>
		</tr>
		<tr>
			<td colspan="4">Extraction Equipment</td>
		</tr>
		<tr>
			<td>Rotavapor</td>
			<td>Buchi</td>
			<td>R-300</td>
			<td></td>
		</tr>
		<tr>
			<td>Shimadzu UV-1800 UV/Visible Scanning Spectrophotometer</td>
			<td>Cole Parmer</td>
			<td>T-83400-20</td>
			<td>Available for other vendors as well</td>
		</tr>
		<tr>
			<td>Ultrasonic Unit</td>
			<td>Elma</td>
			<td>TI-H-20</td>
			<td>Available for other vendors as well</td>
		</tr>
		<tr>
			<td>Vacuum pump</td>
			<td>Buchi</td>
			<td>V-100</td>
			<td></td>
		</tr>
	</tbody>
</table>

rapid model to evaluate the anti-obesity potential of a combination of syzygium aromaticum (clove) and cuminun cyminum (cumin) on c57bl6/j mice fed high-fat diet

Several studies have demonstrated that the phytochemical contents of plants are potential anti-obesity agents. In this study we examine the effect of using a combination of dry buttons from Syzygium aromaticum and seeds from Cuminum cyminum (CC) on C57BL6/J mice induced with obesity via high-fat-diet (HFD). The aim of this study is to demonstrate that the method proposed in the study reduced obesity significantly after several weeks of experimentation. The extract from both plants was extracted using ultrasound to enhance the extraction of phytochemicals. Optimum extraction conditions were obtained with ethanol as follows: 50:50 v/v water with an ultrasound power of 300 W, and ultrasonication time of 30 minutes. The simultaneous administration of the CC extract in HFD for 5 weeks led to the regulation of lipid profiles (cholesterol and triglycerides), reduction of food intake, weight gain, adipose tissue and liver weight. Findings obtained by this obese model indicate that CC extract can prevent obesity. Compared with the traditional 16 weeks method (8 weeks to get fat, and 8 weeks to lose weight), similar results were obtained in the present study obese model in less time of experimentation.

Ultrasonic-assisted extraction 
The optimum extraction condition for the extraction was ethanol:water (50:50, v/v) with an ultrasound power of 300 W and an ultrasonication time of 30 min. This way, the extraction was faster than conventional extraction methods (Table 2). The extract showed an intense absorption peak at 320 nm in UV-visible spectra (Figure 1).
Food intake and body weight 
The changes in the body weight o...

Watch this Scientific Journal Video about Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum Clove and Cuminun cyminum Cumin on C57BL6/j Mice Fed High-Fat Diet 

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum Clove and Cuminun cyminum Cumin on C57BL6/j Mice Fed High-Fat Diet

This work presents a protocol to explore the anti-obesity effect of two plants used together, for a 5-week duration. There was a combined administration of the extract and high-fat-diet (HFD) in the obese mice. The method can promote the benefits of plants in the treatment of obesity.

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet

Several studies have demonstrated that the phytochemical contents of plants are potential anti-obesity agents. In this study we examine the effect of ...

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