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Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

DOI :

10.3791/62146-v

9:38 min

February 2nd, 2021

February 2nd, 2021

3,300 Views

1Vector Biology Department, Liverpool School of Tropical Medicine, 2Department of Microbiology & Molecular Genetics, University of California, 3Department of Molecular Biology & Biochemistry, University of California

The protocol describes how to achieve site-directed modifications in the genome of Anopheles malaria mosquitoes using the φC31 system. Modifications described include both the integration and the exchange of transgenic cassettes in the genome of attP-bearing docking lines.

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Site directed Integration

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