Primary Retinal Ganglion Cell Culture Derived from Zebrafish Embryos
4:45
In Vitro ROS Imaging of Cultured RGC Neurons
6:13
In Vitro ROS Imaging of Developing Embryos
7:40
Results: H2O2 Imaging in Cultured and in Vivo Zebrafish RGCs with roGFP2-Orp1
8:53
Conclusion
Transcript
Our protocol describes the use of a hydrogen peroxide biosensor in cultured zebrafish neurons and larvae. This protocol will help researchers to dissect the role of reactive oxygen species in normal physiology and pathophysiology. The main advanta
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This protocol describes the use of a genetically encoded hydrogen peroxide (H2O2)-biosensor in cultured zebrafish neurons and larvae for assessing the physiological signaling roles of H2O2 during nervous system development. It can be applied to different cell types and modified with experimental treatments to study reactive oxygen species (ROS) in general development.