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TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

DOI :

10.3791/62198-v

7:05 min

February 26th, 2021

February 26th, 2021

4,283 Views

1Dept. Cell Chemical Biology, Leiden University Medical Center, 2Oncode Institute, Leiden University Medical Center

We describe methods to investigate TGF-β2-induced EndMT in endothelial cells by observing cell morphology changes and examining the expression EndMT-related marker changes using immunofluorescence staining. CRISPR/Cas9 gene editing was described and used to deplete the gene encoding Snail to investigate its role in TGF-β2-induced EndMT.

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TGF beta

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