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DOI :
10.3791/62198-v
•
7:05 min
February 26th, 2021
Chapters
0:05
Introduction
1:39
Transforming Growth Factor-β2 (TGF-β2)-Induced Endothelial to Mesenchymal Transition (EndMT)
3:10
EndMT-Related Marker Change Evaluation
5:00
Results: Representative TGF-β2-Induced EndMT in MS-1 Cells
6:17
Conclusion
我们通过观察细胞形态变化和使用免疫荧光染色检查表达 EndMT 相关标记变化来描述在内皮细胞中研究 TGF-β2 诱导 EndMT 的方法。CRISPR/Cas9基因编辑被描述并用于耗尽编码蜗牛的基因,以调查其在TGF-+2诱发的EndMT中的作用。
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