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06:24 min
March 12th, 2021
DOI :
10.3791/62239-v
Chapters
0:04
Introduction
0:47
Cell Barcoding
3:23
Paired scRNA-seq and scATAC-seq
4:40
Results: Multiplexed Analysis Using scRNA-Seq and scATAC-Seq for Characterization of Cell Origin, Phenotype and Gene Regulatory Networks
5:45
Conclusion
Transcript
该协议将使使用单细胞RNA-seq进行初始表型分析成为可能。并且,当与单细胞ATAC-seq配对时,基因调控网络的重建。使用MULTI-seq可降低初始研究的成本,并且固定单细胞RNA-seq和单细胞ATAC-seq的配对样品可以对基因调控网络进行时程分析。
研究人员可以利用该协议来了解控制发育和疾病进展的遗传网络。首先,向每个样品中加入20微升锚定条形码溶液,然后移液混合。在冰上孵育五分钟。
然后向每个样品中加入20微升共锚溶液,并在冰上再孵育五分钟。向每个样品中
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Summary
在这里,作者展示了MULTI-seq用于表型的效用,以及随后配对的scRNA-seq和scATAC-seq在表征视网膜转录组和染色质可及性图谱方面的效用。
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