Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome
Turner syndrome is a rare condition associated with a completely or partially missing X chromosome. The syndrome is characterized by infertility, short stature, cardiovascular disorders, and neurocognitive defects. In some cases, it causes fetal d
Sign in or start your free trial to access this content
This protocol describes the generation of integration free iPSCs from fetal tissue fibroblasts through delivery of episomal plasmids by nucleofection followed by description of methods used for iPSC characterization and neuronal differentiation.