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06:54 min
June 3rd, 2021
DOI :
10.3791/62268-v
Chapters
0:00
Introduction
1:49
Preparation of Buffers and Reagents
2:02
Preparation of Samples
2:56
KCl-mediated Depolarization of Isolated Synaptic Terminals
3:21
Fixation and Phalloidin Staining of Samples
4:40
Fluorometric Analysis and Light Scattering
5:20
Data Analysis
6:19
Conclusion
Transcript
Actin 是细胞骨骼的主要成分,对细胞形态和生理学的几个方面(包括神经元)进行了批判性调节。Actin 发生在其两种形式之间的平衡中,单体球状 G-actin 或聚合细丝 F-actin。作用素的聚合状态通过作用结合蛋白以及自身的转化后调节在多个层面受到严格监管。
在内在突触中,F-actin 水平的动态变化是一个关键的调节事件,可控制突触前和后终端的信号和可塑性。毫不奇怪,行为功能障碍与几个神经病理状态有关。最近的进步已经导致在神经元生理学和病理生理学方面拥有丰富的行为素知
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Summary
我们报告了一种简单、省时和高通量荧光光谱分析,用于从啮齿动物和人类受试者脑组织 中对 活体细丝进行定量。
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