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DOI :
10.3791/62274-v
•
11:55 min
May 28th, 2021
Chapters
0:04
Introduction
1:09
Preparation of the Cryo-Stage
2:08
Transfer of the Sample Storage Box into the Cryo-Stage
3:34
Stage Docking and Focusing
5:03
Brightfield Mosaic Acquisition and Identification of Areas of Interest
6:48
Data Collection
8:59
After Imaging
9:38
Results: Cryo-structured Illumination Microscopic Imaging
11:00
Conclusion
이 프로토콜은 극저온 구조조명 현미경을 사용하여 생물학적 냉동 보존 샘플을 이미지화하는 방법을 보여줍니다. 우리는 U2OS 세포의 세포 골격을 화상 진찰하여 방법론을 보여줍니다.
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