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Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

3.9K Views

11:55 min

May 28th, 2021

May 28th, 2021

3,927 Views

0:04

Introduction

1:09

Preparation of the Cryo-Stage

2:08

Transfer of the Sample Storage Box into the Cryo-Stage

3:34

Stage Docking and Focusing

5:03

Brightfield Mosaic Acquisition and Identification of Areas of Interest

6:48

Data Collection

8:59

After Imaging

9:38

Results: Cryo-structured Illumination Microscopic Imaging

11:00

Conclusion

Transcript

This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells.

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