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06:38 min
April 21st, 2021
DOI :
10.3791/62298-v
Chapters
0:04
Introduction
0:40
Generation of Human Retinal Organoids (RO)
5:02
Results: Human Retinal Organoid Generation
6:07
Conclusion
Transcript
这是视网膜疾病的模型,模拟体内视网膜。在某种程度上,它可以取代动物实验。该方案针对视网膜类器官中感光器前体的质量和数量进行了优化,感光器是几种不可逆失明的关键。
首先在无饲养层条件下培养人类胚胎干细胞或hESC。为此,用一毫升每毫升试剂A的100微克涂覆六孔板的一个孔,并将板在37摄氏度下孵育至少半小时。解冻一百万hESC的等分试样,并以200xg离心五分钟。
除去上清液后,将细胞接种到先前包被的平板中,并在大约四天达到约80%汇合度后与两毫升试剂B.Passage
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Summary
使用自组织方法,我们开发了一种添加COCO的协议,可以显着增加光感受器的产生。
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