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14:59 min
December 11th, 2021
DOI :
10.3791/62315-v
Chapters
0:04
Introduction
0:32
Filtration Procedure for Seawater Dissolved and Particulate Lipids
1:53
Liquid-Liquid Extraction of Seawater or Liquid Samples
3:23
Extraction Protocol for Solids (Modified Folch et al. Extraction)
6:17
Developing and Conditioning Sequences for Separation of Aquatic Lipid Classes by Rod TLC
10:11
FAME Derivatization with H2SO4 in MeOH
13:17
Representative Results
14:30
Conclusion
Transcript
这里描述的方法涉及可操作上定义为海洋脂质的化合物。该定义的基础是它们适合在非极性有机溶剂中进行液液萃取,这提供了一种方便的方法将它们与水基质中的其他化合物分离。它们的疏水性有助于它们从海水或生物标本中分离出来,以及它们的富集和去除盐和蛋白质。
对于海水样品,第一步通常涉及通过过滤分离成操作上定义的溶解和颗粒级分。颗粒部分是由过滤器保留的,孔径对于定义截止值很重要。使用脂质清洁镊子,将47毫米玻璃纤维过滤器放在脂质清洁过滤系统上, 该过滤器已经灰化。
取样并轻轻旋转以
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Summary
该协议用于测定海水和生物标本中的脂质。滤液中的脂质在固体的情况下用氯仿或氯仿和甲醇的混合物提取。脂类通过棒状薄层色谱法和火焰电离检测来测量,其总和给出了总脂质含量。
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