Ex Vivo T Cell Isolation from Peripheral Blood Mononuclear Cells (PBMCs)
2:47
T Cell Stimulation and T Cell Expansion
3:54
Transduction of Lentiviruses on Stimulated T Cells
5:10
Surface Staining of B Cell Maturation Antigen (BCMA)-CAR Using Goat Anti-Mouse IgG (H+L) and Intracellular Staining of NIS Using Anti-Human ETNL NIS
7:18
NIS+BCMA-CAR T Cell In Vivo Imaging Using BCMA+OPM-2 Xenograft Mouse Model
7:57
Results: T Cell Growth Kinetics and In Vivo Cell Trafficking Assay
9:08
Conclusion
Transcript
There is no clinically validated chimeric antigen receptor T-cell imaging platform to date. The sodium-iodide symporter represents a sensitive and clinically relevant reporter to image Car T-cells by PET scan. TLP PET imaging of NIS CAR T-cell all
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This protocol describes the methodology for non-invasively tracking T cells genetically engineered to express chimeric antigen receptors in vivo with a clinically available platform.