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JoVE Journal

Biochemistry

This content is Open Access.

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

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10:32 min

April 23rd, 2021

April 23rd, 2021

2,469 Views

0:04

Introduction

1:15

Crystal Harvesting

1:58

Loading of SH from Combipuck to the Transfer Block

3:44

Loading of Transfer Blocks into the Vacuum Vessel

8:08

Results: Diffraction Images and Structure Solution of Thaumatin

9:41

Conclusion

Transcript

Here, we present a protocol for cryogenic sample preparation and transfer of crystals into the vacuum endstation on beamline I23 at Diamond Light Source, for long-wavelength macromolecular X-ray crystallography experiments.

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