May 18th, 2022
•We have developed a novel loss-of-function approach that involves the introduction and genomic integration of artificial micro-RNA sequences into chick embryos by using in ovo electroporation and the Tol2 transposon system. This technique provides a robust and stable gene knockdown methodology for studies of gene function during development.
Tags
Related Videos
Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication
Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
In ovo Expression of MicroRNA in Ventral Chick Midbrain
Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
Using the Electroretinogram to Assess Function in the Rodent Retina and the Protective Effects of Remote Limb Ischemic Preconditioning
Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved