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07:36 min
June 12th, 2021
DOI :
10.3791/62442-v
Chapters
0:04
Introduction
0:56
Ectopic Tumor Implantation of B16F10-OVA Cells in the Inguinal Region of Mice
2:23
Adoptive Transfer of Congenically Marked OT-I T Cells into Tumor-Bearing Mice
3:26
Dissect Tumor Mass from Tumor-Bearing Donor Mice
4:31
Subcutaneous Transplantation of Donor-Derived Tumor onto the Tumor-Matched Recipient Mice
6:14
Results: For Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
7:04
Conclusion
Transcript
该方法可以帮助回答肿瘤免疫学中的关键问题,包括外周组织中是否存在肿瘤特异性免疫细胞的补充,肿瘤微环境中新浸润的免疫细胞与已经存在的免疫细胞之间的过渡,以及它们对免疫治疗的反应。这是一种方便且易于遵循的技术,用于区分外围来源的细胞和肿瘤浸润的免疫细胞,并在纵向测定中跟踪这些细胞的动态,表型和功能变化。首先将100微升制备的B16F10-OVA细胞悬浮液取出到1mL结核菌素注射器中。
点击枪管将所有气泡移动到顶部,然后轻轻推动柱塞以去除气泡。限制鼠标露出腹部。用小指按压左后腿,收紧
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Summary
在这里,我们提出了一种肿瘤移植方案,用于表征小鼠肿瘤模型中肿瘤固有和外周来源的肿瘤浸润淋巴细胞。通过流式细胞术特异性追踪受体来源的免疫细胞的流入,揭示了抗肿瘤免疫反应期间这些细胞表型和功能变化的动力学。
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