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DOI :
10.3791/62494-v
September 8th, 2021
Chapters
0:04
Introduction
0:44
Bone Marrow Collection and Fixation
1:30
Embedding Bone Marrow in Agarose
2:11
Embedding Bone Marrow in Resin
3:38
Ultrathin Sectioning
4:24
Toluidine Blue Staining
4:54
Heavy Metal Staining
5:31
Transmission Electron Microscopy (TEM)
6:02
Results: In Situ Ultrastructure Exploration of Murine Megakaryopoiesis
7:33
Conclusion
여기서, 우리는 투과 전자 현미경 검사법(TEM)을 사용하여 시투에서 거대 카르요세포의 초구조를 분석하는 프로토콜을 제시한다. 뮤린 뼈 졸은 수집, 고정, 에폭시 수지에 내장및 초박형 섹션에서 절단됩니다. 대조 염색 후, 골수는 120 kV에서 TEM 현미경으로 관찰된다.
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