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2.7K Views
•
07:53 min
August 26th, 2021
DOI :
10.3791/62511-v
Chapters
0:05
Introduction
0:53
Cell Embedding in Methylcellulose Hydrogel
3:03
Cell Resuspension for Proplatelet Analysis
4:24
Cell Fixation and Retrieval for Future Analyses
5:16
Results: Megakaryocyte Characteristics and Proplatelet Quantification According to Culture Conditions
7:08
Conclusion
Transcript
该协议允许体外评估天然巨核细胞在骨髓中暴露于的禁闭和中等硬度对其行为和成熟的影响。该技术的主要优点是简化的模型,消除了细胞 - 细胞和细胞基质相互作用,并专注于机械方面。应严格遵守良好的实验室规范和无菌工作条件。
事实上,即使是水凝胶的轻微污染也会对巨核细胞分化产生巨大影响。为了获得单孔水凝胶细胞培养物,首先在室温下解冻两个一毫升3%甲基纤维素等分试样,然后通过首先吸取一毫升然后将其全部排出来涂覆一毫升的Luer锁定注射器。接下来,使用相同的注射器和针头,从新鲜的等分试样中吸取
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Summary
现在人们承认,细胞的三维环境可以在它们的行为,成熟和/或分化中发挥重要作用。该协议描述了一个三维细胞培养模型,旨在研究物理遏制和机械约束对巨核细胞的影响。
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