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3.8K Views
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08:05 min
November 6th, 2021
DOI :
10.3791/62526-v
Chapters
0:03
Introduction
0:57
Protoplast Extraction
1:49
Small-molecule Calcium Ion Fluorescence Staining
4:46
Protoplast Viability Assay
5:48
Results
7:21
Conclusion
Transcript
钙作为一种重要的矿物质元素,在苹果等树果的生长和质量控制中起着关键作用,它能调节贮藏过程中的硬度、含糖量、储存期和生理紊乱。钙成像是检测细胞质中钙离子动态变化的最西方式。小分子荧光指标,如荧光-4/AM具有钙离子特异性选择性,可以通过酯化孵化进行非侵入性加载,这种孵化具有灵活性、快速性和非细胞毒性。
该协议将引入一种将小分子化学荧光试剂荧光-4/AM加载到植物原质中的方法。前列提取将 0.5 毫升酶溶液加入 1.5 毫升离心机管中。选择一个健康成熟的苹果,然后切成10倍5倍1立
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Summary
苹果浆细胞的分离原体装有荧光钙试剂,以检测细胞质Ca2+ 浓度。
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