Name: minimizing post-infusion portal vein bleeding during intrahepatic islet transplantation in mice
Uploaded: 2021-05-10T15:00:00
Description: Watch this Scientific Journal Video about Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice at JoVE.com

这项研究得到了退伍军人事务部（VA-ORD BLR&D Merit I01BX004536）和美国国立卫生研究院拨款#1R01DK105183，DK120394，DK118529，HW的支持。我们要感谢Michael Lee先生和Lindsay Swaby女士的语言编辑

所有程序均在南卡罗来纳医科大学和查尔斯顿拉尔夫·约翰逊医疗中心的机构动物护理和使用委员会的批准下进行。
1. 使用链脲佐菌素（STZ）诱导糖尿病
<ol><li>受体小鼠制剂：<ol>
<li>单独称量所有小鼠的体重。</li>
		<li>使用血糖仪检查尾静脉血液样本中的血糖水平。</li>
	</ol>
</li>
	<li>三种不同场景的 STZ 剂量测定：<ol>
<li>对于患有脂肪肝疾病的小鼠，连续5天注射一剂STZ [40mg ...

<ol>
	<li>Pellegrini, S., Cantarelli, E., Sordi, V., Nano, R., Piemonti, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+state+of+the+art+of+islet+transplantation+and+cell+therapy+in+type+1+diabetes.">The state of the art of islet transplantation and cell therapy in type 1 diabetes.</a> Acta Diabetology. 53 (5), 683-691 (2016).</li><li>Ballinger, W. F., Lacy, P. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantation+of+intact+pancreatic+islets+in+rats.">Transplantation of intact pancreatic islets in rats.</a> Surgery. 72 (2), 175-186 (1972).</li><li>Wright, J. R., Hauptfeld, V., Lacy, P. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+Ia+antigen+expression+on+murine+islet+parenchymal+cells+does+not+diminish+islet+allograft+survival.">Induction of Ia antigen expression on murine islet parenchymal cells does not diminish islet allograft survival.</a> American Journal of Pathology. 134 (2), 237-242 (1989).</li><li>Toyofuku, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Natural+killer+T-cells+participate+in+rejection+of+islet+allografts+in+the+liver+of+mice.">Natural killer T-cells participate in rejection of islet allografts in the liver of mice.</a> Diabetes. 55 (1), 34-39 (2006).</li><li>Goss, J. A., Nakafusa, Y., Finke, E. H., Flye, M. W., Lacy, P. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+tolerance+to+islet+xenografts+in+a+concordant+rat-to-mouse+model.">Induction of tolerance to islet xenografts in a concordant rat-to-mouse model.</a> Diabetes. 43 (1), 16-23 (1994).</li><li>Hara, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+mouse+model+for+studying+intrahepatic+islet+transplantation.">A mouse model for studying intrahepatic islet transplantation.</a> Transplantation. 78 (4), 615-618 (2004).</li><li>Shapiro, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Islet+transplantation+in+seven+patients+with+type+1+diabetes+mellitus+using+a+glucocorticoid-free+immunosuppressive+regimen.">Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen.</a> New England Journal of Medicine. 343 (4), 230-238 (2000).</li><li>Wang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alpha-1+antitrypsin+enhances+islet+engraftment+by+suppression+of+instant+blood-mediated+inflammatory+reaction.">Alpha-1 antitrypsin enhances islet engraftment by suppression of instant blood-mediated inflammatory reaction.</a> Diabetes. 66 (4), 970-980 (2017).</li><li>Gou, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alpha-1+antitrypsin+suppresses+macrophage+activation+and+promotes+islet+graft+survival+after+intrahepatic+islet+transplantation.">Alpha-1 antitrypsin suppresses macrophage activation and promotes islet graft survival after intrahepatic islet transplantation.</a> American Journal of Transplantation. , (2020).</li><li>Contreras, J. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Activated+protein+C+preserves+functional+islet+mass+after+intraportal+transplantation:+A+novel+link+between+endothelial+cell+activation,+thrombosis,+inflammation,+and+islet+cell+death.">Activated protein C preserves functional islet mass after intraportal transplantation: A novel link between endothelial cell activation, thrombosis, inflammation, and islet cell death.</a> Diabetes. 53 (11), 2804-2814 (2004).</li><li>Moberg, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Production+of+tissue+factor+by+pancreatic+islet+cells+as+a+trigger+of+detrimental+thrombotic+reactions+in+clinical+islet+transplantation.">Production of tissue factor by pancreatic islet cells as a trigger of detrimental thrombotic reactions in clinical islet transplantation.</a> Lancet. 360 (9350), 2039-2045 (2002).</li><li>Melzi, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intrahepatic+islet+transplant+in+the+mouse:+functional+and+morphological+characterization.">Intrahepatic islet transplant in the mouse: functional and morphological characterization.</a> Cell Transplantation. 17 (12), 1361-1370 (2008).</li><li>Wang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Donor+treatment+with+carbon+monoxide+can+yield+islet+allograft+survival+and+tolerance.">Donor treatment with carbon monoxide can yield islet allograft survival and tolerance.</a> Diabetes. 54 (5), 1400-1406 (2005).</li><li>Desai, C. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+liver+histopathology+on+islet+cell+engraftment+in+the+model+mimicking+autologous+islet+cell+transplantation.">Effect of liver histopathology on islet cell engraftment in the model mimicking autologous islet cell transplantation.</a> Islets. 9 (6), 140-149 (2017).</li><li>Cui, W., Angsana, J., Wen, J., Chaikof, E. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Liposomal+formulations+of+thrombomodulin+increase+engraftment+after+intraportal+islet+transplantation.">Liposomal formulations of thrombomodulin increase engraftment after intraportal islet transplantation.</a> Cell Transplantation. 19 (11), 1359-1367 (2010).</li><li>Cui, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thrombomodulin+improves+early+outcomes+after+intraportal+islet+transplantation.">Thrombomodulin improves early outcomes after intraportal islet transplantation.</a> American Journal of Transplantation. 9 (6), 1308-1316 (2009).</li><li>Proto, C., Grasso, G., Fassio, P. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hepatoparenchymal+clearance+of+indocyanine+green+in+infectious+hepatitis.">Hepatoparenchymal clearance of indocyanine green in infectious hepatitis.</a> Giornale di Malattie Infettive e Parassitarie. 20 (9), 845-851 (1968).</li><li>Cabral, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Purification+of+hepatocytes+and+sinusoidal+endothelial+cells+from+mouse+liver+perfusion.">Purification of hepatocytes and sinusoidal endothelial cells from mouse liver perfusion.</a> Journal of Visualized Experiments. (132), e56993 (2018).</li><li>Khatri, R., Hussmann, B., Rawat, D., Gurol, A. O., Linn, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraportal+transplantation+of+pancreatic+islets+in+mouse+model.">Intraportal transplantation of pancreatic islets in mouse model.</a> Journal of Visualized Experiments. (135), e57559 (2018).</li><li>Wang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autologous+mesenchymal+stem+cell+and+islet+cotransplantation:+Safety+and+efficacy.">Autologous mesenchymal stem cell and islet cotransplantation: Safety and efficacy.</a> Stem Cells Translational Medicine. 7 (1), 11-19 (2018).</li></ol>

在这项研究中，已经证明了两种改进的程序，可以预防出血并可能降低小鼠IIT期间的小鼠死亡率。这项研究使研究人员能够可视化胰岛移植模型，该模型在研究移植后血液介导的即时炎症反应方面是独一无二的。IIT模型是研究胰岛细胞存活和胰岛移植引起的肝缺血性损伤的独特模型19。在这里，我们根据以前的研究改进了程序，并降低了早期并发症诱导的小鼠死亡率。方法<sup cla...

通过门静脉进行门静脉门内胰岛移植 （IIT） 是临床环境中最常用的人胰岛移植方法。小鼠IIT模型为研究胰岛移植和测试可以提高胰岛移植疗效的有希望的介入方法提供了很好的机会1。IIT在20世纪70年代首次被描述，并被几个团体使用1，2，3，4，5。在20006年人类胰岛移植取得突破后，它重新流行起来，7。 然而，大多数胰岛移植研究使用肾胶囊作为实验性胰岛移植的首选部位，因为它很容易成功。相反，IIT在技术上更具挑战性，用于胰岛移植研究的频率较低8，9。然而，与IIT不同的是，移植在肾囊下的胰岛不会立即发生以血栓形成，炎症和肝组织缺血为特征的血液介导的炎症反应，因此比移植到肝脏中的胰岛具有更好的功能。因此，肾胶囊模型可能无法完全模仿胰岛移植中胰岛遇到的应激10，11，12。
小鼠IIT的主要并发症之一是移植后注射部位出血，这可能导致不同小鼠品系死亡率的10-30%12。在本文中，已经开发了两种改进的方法，以更快，更安全地止血并降低IIT后的小鼠死亡率。这些精致细节的视觉演示将帮助研究人员确定这一技术上具有挑战性的程序的关键步骤。此外，通过对含移植胰岛的苏木精和曙红（H&E）染色的肝组织（整个切片）的组织学检查来确定胰岛移植物在受体肝脏中的位置。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>10% Neutral buffered formalin v/v</td>
			<td>Fisher Scientific</td>
			<td>23426796</td>
			<td></td>
		</tr>
		<tr>
			<td>1 mL Syringe with needle</td>
			<td>AHS</td>
			<td>AH01T</td>
			<td></td>
		</tr>
		<tr>
			<td>20 mL Syringe</td>
			<td>BD</td>
			<td>301031</td>
			<td></td>
		</tr>
		<tr>
			<td>25G x 5/8&#34; hypodermic needles</td>
			<td>BD</td>
			<td>305122</td>
			<td></td>
		</tr>
		<tr>
			<td>Alcohol prep pads, sterile</td>
			<td>Fisher Scientific</td>
			<td>22-363-750</td>
			<td></td>
		</tr>
		<tr>
			<td>Animal Anesthesia system</td>
			<td>VetEquip, Inc.</td>
			<td>901806</td>
			<td></td>
		</tr>
		<tr>
			<td>Buprenorphine hydrochloride, injection</td>
			<td>Par Sterile Products, LLC</td>
			<td>NDC 42023-179-05</td>
			<td></td>
		</tr>
		<tr>
			<td>Centrifuge tubes, 15 mL</td>
			<td>Fisher Scientific</td>
			<td>0553859A</td>
			<td></td>
		</tr>
		<tr>
			<td>CMRL-1066</td>
			<td>Corning</td>
			<td>15110CV</td>
			<td></td>
		</tr>
		<tr>
			<td>DMEM</td>
			<td>Corning</td>
			<td>10013CV</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol, absolute (200 proof), molecular biology grade</td>
			<td>Fisher Scientific</td>
			<td>BP2818500</td>
			<td></td>
		</tr>
		<tr>
			<td>Extra fine Micro Dissecting scissors 4&#8221; straight sharp</td>
			<td>Roboz Surgical Instrument Co.</td>
			<td>RS-5882</td>
			<td></td>
		</tr>
		<tr>
			<td>Fetal bovine serum (FBS)</td>
			<td>Corning</td>
			<td>35011CV</td>
			<td></td>
		</tr>
		<tr>
			<td>FreeStyle&#160; Glucose meter</td>
			<td>Abbott</td>
			<td>Lite</td>
			<td></td>
		</tr>
		<tr>
			<td>FreeStyle Blood Glucose test strips</td>
			<td>Abbott</td>
			<td>Lite</td>
			<td></td>
		</tr>
		<tr>
			<td>Gelfoam (absorbable gelatin sponge, USP)</td>
			<td>Pharmacia &#38; Upjohn Company</td>
			<td>34201</td>
			<td></td>
		</tr>
		<tr>
			<td>Graefe forceps 4&#8221; extra delicate tip</td>
			<td>Roboz Surgical Instrument Co.</td>
			<td>RS-5136</td>
			<td></td>
		</tr>
		<tr>
			<td>Heated pad</td>
			<td>Amazon</td>
			<td>B07HMKMBKM</td>
			<td></td>
		</tr>
		<tr>
			<td>Hegar-Baumgartner Needle Holder 5.25&#8221;</td>
			<td>Roboz Surgical Instrument Co.</td>
			<td>RS-7850</td>
			<td></td>
		</tr>
		<tr>
			<td>Insulin syringe with 27-gauge needle</td>
			<td>BD</td>
			<td>879588</td>
			<td></td>
		</tr>
		<tr>
			<td>Iodine prep pads</td>
			<td>Fisher Scientific</td>
			<td>19-027048</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>Piramal Critical Care</td>
			<td>NDC 66794-017-25</td>
			<td></td>
		</tr>
		<tr>
			<td>Penicillin/streptomycin (P/S)</td>
			<td>HyClone</td>
			<td>SV30010</td>
			<td></td>
		</tr>
		<tr>
			<td>Polypropylene Suture 4-0</td>
			<td>Med-Vet International</td>
			<td>MV-8683</td>
			<td></td>
		</tr>
		<tr>
			<td>Polypropylene Suture 5-0</td>
			<td>Med-Vet International</td>
			<td>MV-8661</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium chloride, 0.9% intravenous solution</td>
			<td>VWR</td>
			<td>2B1322Q</td>
			<td></td>
		</tr>
		<tr>
			<td>Streptozocin (STZ)</td>
			<td>Sigma</td>
			<td>S0130</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical drape, sterile</td>
			<td>Med-Vet International</td>
			<td>DR1826</td>
			<td></td>
		</tr>
		<tr>
			<td>Tissue Cassette</td>
			<td>Fisher Scientific</td>
			<td>22-272416</td>
			<td></td>
		</tr>
	</tbody>
</table>

minimizing post-infusion portal vein bleeding during intrahepatic islet transplantation in mice

虽然肝脏目前在临床环境中被接受为人类胰岛的主要移植部位，但在大多数啮齿动物临床前胰岛移植研究中，胰岛被移植到肾囊下。该模型是常用的，因为小鼠肝内胰岛移植在技术上具有挑战性，并且很大比例的小鼠可能死于手术并发症，特别是移植后注射部位的出血。在这项研究中，证明了两种可以最大限度地减少输注后门静脉出血发生率的程序。第一种方法将可吸收的止血明胶海绵应用于注射部位，第二种方法涉及首先穿透胰岛注射针穿过脂肪组织，然后通过使用脂肪组织作为物理屏障进入门静脉以止血。这两种方法都可以有效防止出血引起的小鼠死亡。介绍了胰岛分布和移植后胰岛血栓形成证据的整个肝脏切片，这是肝内胰岛移植的典型特征。这些改进的方案改进了肝内胰岛移植程序，并可能有助于实验室建立程序，以研究胰岛存活率和临床前功能。

我们通过门静脉进行了同源和异种胰岛移植。在两种胰岛移植模型中都以剂量依赖性方式观察到胰岛移植物功能。在使用C57BL / 6小鼠的同源胰岛移植模型中，移植250个小岛导致小鼠恢复到高血糖之前暂时性正常血糖。接受500个胰岛的小鼠在移植后30天后达到并保持正常血糖（图2A）。两组小鼠均显示体重增加（图2B）。
同样，在人?...

Watch this Scientific Journal Video about Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice at JoVE.com

Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice

在这里，我们介绍了在小鼠中成功进行门内胰岛移植的精细外科手术，这是一种临床相关但技术上具有挑战性的外科手术。

尽量减少小鼠肝内胰岛移植期间输注后门静脉出血

Although the liver is currently accepted as the primary transplantation site for human islets in clinical settings, islets are transplanted under the ...

肝脏是人类胰岛临床环境中移植的主要部位。小鼠肝内胰岛移植在技术上具有挑战性，并且很大比例的小鼠可能死于手术并发症，尤其是出血。在这项研究中，我们展示的两种方法都可以有效预防出血引起的小鼠死亡。我们的方法可以通过允许将癌细胞，间充质干细胞和肝细胞直接注射到肝脏中来应用于肝癌和其他肝脏疾病的研究。首先，通过轻柔的抓挠分离人类胰岛文化。使用1立方厘米注射器将300至350个小岛掰入冰上的无菌1.5毫升微量离心管中。当所有胰岛都被采摘后，离心管10秒钟以沉淀细胞。除少量上清液外，弃去所有上清液以防止沉淀丢失，并将胰岛重悬于含有5%BSA的200微升HBSS中。将重悬的胰岛装入5毫升胰岛素注射器中，并将注射器针端向上转动1分钟，让胰岛在柱塞处沉降。压低柱塞以除去任何气泡，在注射器中保持约100至150微升的溶液，并用轻轻敲击倒注射器以均匀分布胰岛。在确认对踏板反射缺乏反应后，将软膏涂抹在眼睛上以防止干燥。剃掉麻醉小鼠的腹部以去除毛皮，并用三个2%碘和75%酒精的交替擦拭物对手术区域进行消毒。接下来，在腹部做一个1至1.5厘米的切口，打开腹膜腔。在切口周围放置一块无菌纱布，并使用镊子或棉签轻轻地将肠子转移到纱布上。然后用一块盐水浸泡的纱布覆盖肠道并暴露门静脉。要使用凝胶泡沫停止胰岛转移引起的出血，请握住针头，斜面朝下，并将针尖的开口与门静脉壁平行，然后将针头插入门静脉。将柱塞缩回，将20至50微升的血液吸入注射器中，与胰岛混合，然后将胰岛注入门静脉，同时反复压低并缩回柱塞。当所有胰岛都已交付后，在注射部位放置约5×5厘米的凝胶泡沫。使用棉签将凝胶泡沫压向下，同时将针头从门静脉中取出。将凝胶固定在适当的位置约2分钟，滚动凝胶泡沫以确保其覆盖门静脉。一旦出血停止，轻轻地将肠子放回原始位置的腹膜腔，并将500微升温盐水注入腹腔。使用缝合线关闭肌肉和皮肤层。然后将小鼠放在加热垫上的干净笼子中，并进行监测，直到从麻醉中完全恢复，每12小时给予镇痛，加热48小时。为了使用脂肪垫停止胰岛转移引起的出血，在如图所示暴露门静脉后，使用两个棉头将暴露的静脉保持在左侧和右侧，并将脂肪组织垫定位在十二指肠和门静脉之间。在插入门静脉之前，用针尖穿透脂肪垫。如图所示，将胰岛注入门静脉。当所有胰岛都已送达时，用棉头按压脂肪组织约1分钟，同时从静脉中取出针头。然后将肠子放回腹腔，并执行其余的术后护理程序，如所示。胰岛移植的效果是剂量依赖性的，因为在同源胰岛移植后30天转移500个胰岛时观察到正常血糖，但当250个胰岛被递送时，仅观察到短暂的正常血糖，小鼠在移植后10天返回高血糖。两组均观察到体重增加，当人类胰岛移植到糖尿病NOD-SCID小鼠中时观察到相同的反应，当移植更多数量的胰岛当量但不小于数量时观察到正常血糖。大多数在转移后出血的小鼠在手术后死亡，而没有出血的小鼠存活下来。在移植后28天，胰岛素染色显示人胰岛在整个肝脏中的均匀分布，主要靠近血管。除胰岛素外，在移植的人类胰岛内还观察到纤维蛋白和多形核细胞过滤。肝内胰岛移植会在门静脉中产生一个大孔，可能导致出血。使用我们两种改进的程序可以防止出血并降低胰岛移植后的死亡率。门内胰岛移植模型可以模拟临床人胰岛移植。我们改进的程序可以提高受者的存活率，从而允许在移植后进一步研究胰岛功能。

Research

JoVE Journal

Medicine

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

小鼠胰岛的分离和包膜下肾移植的方法

Since the early pioneering work of Ballinger and Reckard demonstrating that transplantation of islets of Langerhans into diabetic rodents could normalize ...

科研

医学

3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System

树脂浇铸和3维成像的小鼠门静脉或肝内胆管系统

In  organs, the correct architecture of vascular and ductal structures is  indispensable for proper physiological function, and the formation and  ...

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation

直接压力监测准确预测在Cryoballoon消融肺静脉阻塞

Cryoballoon  ablation (CBA) is an established therapy for atrial fibrillation (AF).  Pulmonary vein (PV) occlusion is essential for achieving antral ...

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice

皮下及肝内人肝癌裸鼠移植瘤的免疫缺陷小鼠的产生

In  vivo experimental models of hepatocellular carcinoma (HCC) that recapitulate  the human disease provide a valuable platform for research into disease  ...

Heterotopic Auxiliary Rat Liver Transplantation With Flow-regulated Portal Vein Arterialization in Acute Hepatic Failure

在急性肝衰竭异位辅助性大鼠肝移植流量调节门静脉动脉

In acute hepatic failure auxiliary liver transplantation is an interesting alternative approach. The aim is to provide a temporary support until the ...

Heterotopic Cervical Heart Transplantation in Mice

颈椎异位心脏移植小鼠

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

套筒技术在主动脉移植小鼠模型中的应用-教学视频

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the ...

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy ALPPS Procedure

肝分型与门静脉结扎大鼠分期肝切除 (ALPPS) 手术模型的建立

Recent clinical data support an aggressive surgical approach to both primary and metastatic liver tumors. For some indications, like colorectal liver ...

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

肝细胞部分肝切除术后脾移植。小鼠

Partial hepatectomy is a versatile and reproducible method to study liver regeneration and the effect of cell based therapeutics in various pathological ...

Blood Collection Through Subclavian Vein Puncture in Mice

小鼠锁骨下静脉穿刺采血

The mouse is the foremost mammalian model for studying human disease and human health. However, blood sample collection from mice is challenging in ...