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DOI :
10.3791/62544-v
August 7th, 2021
Chapters
0:00
Introduciton
0:54
Cell Harvesting and Lysis
4:13
MNase Digestion and Size Selection of Samples for RIBO-seq
6:17
rRNA Depletion and Alkaline Fragmentation of Samples for RNA-seq
7:07
Library Preparation and Size Selection of cDNA Libraries
8:49
Results: Quality of cDNA Libraries and Reads
11:31
Conclusion
在这里,我们描述了细菌中RIBO-seq样品收集和制备的阶段。根据这些准则对图书馆进行排序,可以获得足够的数据,以便进行全面的生物信息分析。我们目前的协议很简单,使用标准的实验室设备,从裂解到获得库需要七天时间。
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