Sample Preparation #2- Cell Lysis & Medium Exchange
4:28
FCS Calibration
8:48
FCS Measurement
11:45
Representative Results
13:03
Conclusion
Transcript
The best principle of the Fluorescence correlation spectroscopy, FCS, was invented initially in the 1970s. In the 1990s, important and many improvements for FCS was established by combining with a confocal apparatus microscopy. Since then, FCS has
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We here introduce a procedure to measure protein oligomers and aggregation in cell lysate and live cells using fluorescence correlation spectroscopy.